7THW
Crystal Structure of the Soluble Domain of the Putative OmpA -Family Membrane Protein YPO0514 from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.861, 123.099, 54.183 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 49.590 - 2.200 |
| R-factor | 0.2285 |
| Rwork | 0.226 |
| R-free | 0.26880 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.153 | 0.776 |
| Number of reflections | 29832 | 1116 |
| <I/σ(I)> | 18.5 | 1.02 |
| Completeness [%] | 93.8 | 72.1 |
| Redundancy | 4.9 | |
| CC(1/2) | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.3 M Magnesium Formate 0.1 M Tris:HCl pH 8.5 |
