7TH0

Escherichia coli RpnA-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-06-03
Detector	DECTRIS EIGER2 S 4M
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	38.020, 58.270, 38.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.360 - 1.900
R-factor	0.2258
Rwork	0.225
R-free	0.24700
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.360	1.950
High resolution limit [Å]	1.900	1.910
Number of reflections	3582	262
<I/σ(I)>	43.46
Completeness [%]	99.8
Redundancy	13.48
CC(1/2)	1.000	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5M LiCl 0.1M Tris HCl 30% PEG 6K 5 mM Fe(III)Cl