7TGP

E176T/Y188G variant of the internal UBA Domain of HHR23A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 43
Unit cell lengths	31.120, 31.120, 40.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.120 - 1.400
R-factor	0.1613
Rwork	0.161
R-free	0.17470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w2g
RMSD bond length	0.004
RMSD bond angle	0.499
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.120	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	7624	754
<I/σ(I)>	10
Completeness [%]	99.8	99.21
Redundancy	13.6
CC(1/2)	0.997	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	sodium formate