7TFZ
[T:Ag+/Hg2+:T--(pH8.5-pH11; 5s)] Metal-mediated DNA base pair in tensegrity triangle grown at pH 8.5 and soaked in pH 11 for 5s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.224, 107.224, 85.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.740 - 6.130 |
| R-factor | 0.2088 |
| Rwork | 0.203 |
| R-free | 0.21380 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.772 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.610 | 7.510 |
| High resolution limit [Å] | 5.850 | 5.850 |
| Number of reflections | 603 | 418 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 80.1 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.995 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Tris, magnesium sulfate, silver nitrate, mercury chloride |
