7TEM
Crystal Structure of the Putative Exported Protein YPO2471 from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.774, 90.016, 181.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.440 - 1.650 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.18870 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.100 | 0.921 |
| Number of reflections | 106782 | 4253 |
| <I/σ(I)> | 25.9 | 1.43 |
| Completeness [%] | 97.6 | |
| Redundancy | 7 | |
| CC(1/2) | 0.995 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Sodium acetate trihydrate pH 7.0, 20 % (w/v) Polyethylene glycol 3,350 |
