7TDS

Labrum-interacting protein from saliva LIPS-2 (34K-2) from Aedes albopictus, native data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-02
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.966
Spacegroup name	P 43 21 2
Unit cell lengths	109.875, 109.875, 141.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.430 - 2.200
R-factor	0.1874
Rwork	0.186
R-free	0.20790
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.260	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.072	1.426
Rpim	0.035	0.728
Number of reflections	43932	3689
<I/σ(I)>	14.2	1.3
Completeness [%]	98.5	97.8
Redundancy	7.9	8
CC(1/2)	0.999	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	1 M (NH4)2SO4, 1 M LI2SO4, 0.1 M KNO3