7TDR

Labrum-interacting protein from saliva LIPS-2 (34K-2) from Aedes albopictus, selenomethionine derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	109.943, 109.943, 141.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.170 - 2.280
Rwork	0.195
R-free	0.20620
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.170	2.360
High resolution limit [Å]	2.280	2.280
Rmerge	0.104	1.867
Rpim	0.029	0.610
Number of reflections	40218	3896
<I/σ(I)>	19.4
Completeness [%]	99.9	99.9
Redundancy	25.5
CC(1/2)	0.999	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	1 M (NH4)2SO4, 1 M LI2SO4, 0.1 M KNO3