7TCY

The ubiquitin-associated domain of human thirty-eight negative kinase I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	P 21 21 2
Unit cell lengths	53.734, 88.070, 26.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.030 - 1.540
R-factor	0.1899
Rwork	0.189
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.030	1.595
High resolution limit [Å]	1.540	1.540
Rmerge	0.056	0.813
Rmeas	0.059	0.865
Rpim	0.018	0.289
Number of reflections	19047	1846
<I/σ(I)>	19.86	2.48
Completeness [%]	97.6	99.51
Redundancy	10	8.6
CC(1/2)	0.999	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	100 mM Bis-Tris, pH 6.8, 100 mM magnesium formate