7TCD
LOV2-DARPIN fusion: D13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-20 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.504, 47.902, 78.068 |
| Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
| Resolution | 45.070 - 1.700 |
| R-factor | 0.1849 |
| Rwork | 0.181 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tal |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.792 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.070 | 1.970 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | |
| Rmeas | 0.079 | |
| Rpim | 0.041 | |
| Number of reflections | 31319 | 1567 |
| <I/σ(I)> | 10.2 | 1.9 |
| Completeness [%] | 83.5 | 35.9 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.998 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M sodium chloride 28% PEG4000 100 mM sodium citrate, pH 6.0 |
