7TBR

Crystal structure of the TPM domain from the Rhodothermus marinus protein Rhom172_1776

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.885601
Spacegroup name	C 1 2 1
Unit cell lengths	84.780, 37.560, 48.530
Unit cell angles	90.00, 118.15, 90.00

Refinement procedure

Resolution	42.790 - 1.100
R-factor	0.19216
Rwork	0.191
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5anp
RMSD bond length	0.015
RMSD bond angle	1.846
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.790	1.160
High resolution limit [Å]	1.100	1.100
Rmeas	0.039	1.021
Number of reflections	54711	8430
<I/σ(I)>	18.5	1.5
Completeness [%]	99.0	95.4
Redundancy	6.6	5.6
CC(1/2)	0.999	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	12% PEG 6000, 5% isopropanol, 0.1 M Bis-tris-propane pH 8.4