7TBO
LOV2-DARPIN fusion : D12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.356, 71.645, 78.844 |
| Unit cell angles | 68.57, 83.13, 90.05 |
Refinement procedure
| Resolution | 50.010 - 2.320 |
| R-factor | 0.2008 |
| Rwork | 0.196 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7tbn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.691 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.780 | 2.610 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.079 | |
| Rmeas | 0.092 | |
| Rpim | 0.047 | |
| Number of reflections | 22563 | 1129 |
| <I/σ(I)> | 6.6 | 1.6 |
| Completeness [%] | 84.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.995 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM sodium acetate, 17% PEG10000, 20 mM BIS-Tris, pH 5.5 |
