7TBA
Pentraxin - ligand complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.568, 98.172, 160.400 |
| Unit cell angles | 89.08, 89.76, 87.48 |
Refinement procedure
| Resolution | 41.540 - 3.500 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b09 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.540 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 184033 | 5362 |
| <I/σ(I)> | 4.63 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.947 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100mM Tris pH 9.0, 10% PEG 4000, 50mM lithium chloride, 200mM magnesium chloride |
