7TAV
Crystal Structure of the PBP2_YvgL_like protein Lmo1041 from Listeria monocytogene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 32 |
| Unit cell lengths | 113.715, 113.715, 114.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.220 - 2.750 |
| R-factor | 0.2151 |
| Rwork | 0.213 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alpha Fold 2 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.378 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.176 | 1.028 |
| Number of reflections | 42691 | 2126 |
| <I/σ(I)> | 9.58 | 1.54 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.2 | 3 |
| CC(1/2) | 0.962 | 0.393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Calcium Chloride, 20 % (w/v) PEG 3360 |
