7TAE

Crystal Structure of the NPR1-Interacting Domain of TGA3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	68.390, 101.740, 83.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.020 - 1.500
R-factor	0.1897
Rwork	0.189
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tad
RMSD bond length	0.006
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.020	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.048
Rmeas	0.050
Rpim	0.014
Number of reflections	47111	4631
<I/σ(I)>	25.51
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	0.999	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM HEPES (pH 8.0), 75 mM NaCl, 0.5 mM TCEP. 0.05 M sodium acetate pH 5.0, 10% (v/v) MPD 5 mg/mL protein