7TA2
Crystal structure of the human sperm-expressed surface protein SPACA6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-25 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.91165 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.984, 46.522, 167.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.830 - 2.250 |
R-factor | 0.2037 |
Rwork | 0.201 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jk9 |
Data reduction software | DIALS (v2.2.0) |
Data scaling software | Aimless (0.7.4) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.830 | 44.830 | 2.320 |
High resolution limit [Å] | 2.250 | 9.000 | 2.250 |
Rmerge | 0.108 | 0.046 | 0.754 |
Rmeas | 0.116 | 0.050 | 0.815 |
Rpim | 0.041 | 0.018 | 0.303 |
Number of reflections | 11487 | 234 | 1029 |
<I/σ(I)> | 11.7 | ||
Completeness [%] | 99.9 | 99.7 | 100 |
Redundancy | 7.2 | 5.9 | 6.7 |
CC(1/2) | 0.998 | 0.999 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | Ammonium chloride, PEG 3350, FOS-choline-8 |