7T9X

Saccharomyces cerevisiae Pex12 RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	293
Detector technology	CCD
Collection date	2019-06-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.967
Spacegroup name	P 1 21 1
Unit cell lengths	27.578, 32.922, 72.488
Unit cell angles	90.00, 100.72, 90.00

Refinement procedure

Resolution	71.220 - 1.520
R-factor	0.1872
Rwork	0.186
R-free	0.19700
Structure solution method	AB INITIO PHASING
RMSD bond length	0.005
RMSD bond angle	0.838
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.220	4.800
High resolution limit [Å]	1.520	1.520
Rmerge	0.057	0.057
Number of reflections	34282	18534
<I/σ(I)>	36.1
Completeness [%]	97.1	83.8
Redundancy	9.3
CC(1/2)		0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	0.1 M bis-Tris propane, pH 8.5, 0.2 M sodium fluoride, 20% PEG3350