7T99

Crystal structure of engineered CYS-CYS fab dimer CL-205 (LC25)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	52.824, 62.451, 124.018
Unit cell angles	89.99, 98.37, 89.89

Refinement procedure

Resolution	62.450 - 2.650
R-factor	0.1872
Rwork	0.184
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.787
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.450	62.450	2.800
High resolution limit [Å]	2.650	8.400	2.660
Rmerge	0.029	0.015	0.154
Rmeas	0.041	0.021	0.218
Rpim	0.029	0.015	0.154
Total number of observations		2543	11023
Number of reflections	37526	1302	5959
<I/σ(I)>	6.5	11.6	2.3
Completeness [%]	82.9	90.6	90.2
Redundancy	1.8	2	1.8
CC(1/2)	0.999	0.999	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% PEG 3350, 100 mM sodium succinate