7T98
Crystal structure of engineered CYS-CYS fab dimer VL-108 (LC33)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.634, 99.634, 212.675 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.890 - 2.970 |
| R-factor | 0.1962 |
| Rwork | 0.192 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.959 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.890 | 70.890 | 3.900 |
| High resolution limit [Å] | 2.970 | 11.700 | 3.700 |
| Rmerge | 0.248 | 0.049 | 0.787 |
| Rmeas | 0.262 | 0.052 | 0.829 |
| Rpim | 0.083 | 0.017 | 0.257 |
| Total number of observations | 4389 | 20096 | |
| Number of reflections | 17133 | 506 | 1947 |
| <I/σ(I)> | 9.8 | 29.5 | 3.5 |
| Completeness [%] | 99.9 | 99.4 | 100 |
| Redundancy | 9.8 | 8.7 | 10.3 |
| CC(1/2) | 0.994 | 0.999 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1% PEG 2000, 100 mM HEPES pH 8.0 |
