7T91

Crystal structure of Zinc finger motif 1 and 2 of GLI1 DNA binding region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	66.728, 66.728, 65.909
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.790 - 2.050
R-factor	0.194
Rwork	0.192
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gli
RMSD bond length	0.005
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.790	2.100
High resolution limit [Å]	2.050	2.050
Rpim	0.034	0.283
Number of reflections	10542	818
<I/σ(I)>	17.3
Completeness [%]	100.0
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	29-32% PEG3350, 0.1M Bis-Tris pH6.5