7T8P
CRYSTAL STRUCTURE OF T151V CAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-07 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979110 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 101.507, 101.507, 450.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.080 - 1.900 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20410 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5u8x |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 1.542 |
| Number of reflections | 210666 | 32972 |
| <I/σ(I)> | 11.2 | 0.91 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 4 | 3.9 |
| CC(1/2) | 0.998 | 0.343 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.05 M HEPES pH 7 36% sodium polyacrylate 2100 |
