7T8O
Crystal Structure of the Crp/Fnr Family Transcriptional Regulator from Listeria monocytogenes
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97899 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.091, 76.308, 171.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.750 - 2.710 |
| R-factor | 0.2523 |
| Rwork | 0.251 |
| R-free | 0.28500 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.437 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.189 | 0.635 |
| Number of reflections | 15776 | 545 |
| <I/σ(I)> | 10.8 | 1.41 |
| Completeness [%] | 91.7 | 67.8 |
| Redundancy | 4.5 | 2.3 |
| CC(1/2) | 0.996 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Lithium Sulfate, 0.1 M Tris HKL pH 8.5, 20 % (w/v) PEG 4000 |
