7T8N

Crystal structure of the PNAG binding module PgaA-TPR 220-359

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 62
Unit cell lengths	60.670, 60.670, 185.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.001 - 2.850
Rwork	0.235
R-free	0.26430
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.424
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.001	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.121	0.634
Number of reflections	8565	829
<I/σ(I)>	28.8
Completeness [%]	99.4
Redundancy	21.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	15% w/v PEG 8000, 0.1 M Tris pH 7.1, 200 mM MgCl2, and 3% w/v cadaverine