7T89

Light harvesting complex Phycocyanin PC577 from the cryptophyte Hemiselmis pacifica CCMP 706

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953698
Spacegroup name	P 21 21 21
Unit cell lengths	42.116, 95.322, 125.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.280 - 1.000
R-factor	0.1331
Rwork	0.133
R-free	0.14280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lms
RMSD bond length	0.007
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	Aimless (version 0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.280	1.040
High resolution limit [Å]	1.000	1.020
Rmerge	0.061	0.372
Rmeas	0.078
Rpim	0.049	0.330
Number of reflections	253580	7164
<I/σ(I)>	16.6
Completeness [%]	93.4	58.1
Redundancy	4.6	2.3
CC(1/2)	0.993	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG 10K, 15-25% 100 mM HEPES, pH7.5