7T89
Light harvesting complex Phycocyanin PC577 from the cryptophyte Hemiselmis pacifica CCMP 706
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953698 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.116, 95.322, 125.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.280 - 1.000 |
| R-factor | 0.1331 |
| Rwork | 0.133 |
| R-free | 0.14280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | Aimless (version 0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.280 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.020 |
| Rmerge | 0.061 | 0.372 |
| Rmeas | 0.078 | |
| Rpim | 0.049 | 0.330 |
| Number of reflections | 253580 | 7164 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 93.4 | 58.1 |
| Redundancy | 4.6 | 2.3 |
| CC(1/2) | 0.993 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 10K, 15-25% 100 mM HEPES, pH7.5 |
