7T80

Crystal Structure of Mouse Cadherin-23 EC18-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	76.073, 76.073, 201.319
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.106 - 2.301
Rwork	0.200
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wjm 5tfk
RMSD bond length	0.008
RMSD bond angle	1.641
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.106	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	30864	1495
<I/σ(I)>	21.28	2.7
Completeness [%]	100.0	99.9
Redundancy	17.6	13.4
CC(1/2)	0.999	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2 M (NH4)2SO4 25% w/v PEG 3350 0.1 M Tris-HCl 8.5 pH