Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.499, 32.350, 32.260 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 32.255 - 2.300 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.24170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ixj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.255 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.109 | 0.288 |
| Number of reflections | 3487 | 350 |
| <I/σ(I)> | 5.7 | 5 |
| Completeness [%] | 87.2 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.940 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 20% MPD, 120 mM barium chloride, 30 mM sodium cacodylate, equilibrated against 30% MPD |
