7T6W

Crystal structure of Chaetomium Glucosidase I (apo)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	I 2 2 2
Unit cell lengths	139.152, 178.804, 180.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 2.600
R-factor	0.1926
Rwork	0.190
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j5t
RMSD bond length	0.008
RMSD bond angle	1.186
Data reduction software	HKL-2000 (v717.1)
Data scaling software	HKL-2000 (v717.1)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.060	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.170	1.480
Rmeas	0.180
Rpim	0.073	0.655
Number of reflections	68891	6443
<I/σ(I)>	6.98	1.07
Completeness [%]	99.2	93.79
Redundancy	6.4
CC(1/2)	0.991	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	297	0.1 Bis Tris pH 6.5, 1.6-2.0 M Ammonium sulfate