7T6A

Crystal structure of Avr1 (SIX4) from Fusarium oxysporum f. sp. lycopersici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	70.004, 40.343, 81.299
Unit cell angles	90.00, 104.54, 90.00

Refinement procedure

Resolution	39.350 - 1.650
R-factor	0.1706
Rwork	0.169
R-free	0.21090
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.744
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.067	0.990
Rpim	0.047	0.633
Number of reflections	53375	2645
<I/σ(I)>	13.1	1.5
Completeness [%]	99.9	100
Redundancy	5.5
CC(1/2)	0.999	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.5, 17.5% (w/v) PEG 4000