7T69

Crystal structure of Avr3 (SIX1) from Fusarium oxysporum f. sp. lycopersici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	54.861, 80.133, 117.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.270 - 1.680
R-factor	0.1685
Rwork	0.165
R-free	0.21460
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.173
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.270	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.069	0.848
Rmeas		0.951
Rpim	0.034	0.426
Number of reflections	29849	1453
<I/σ(I)>	14.5	1.8
Completeness [%]	99.8	96.8
Redundancy	9.4	9.2
CC(1/2)	0.999	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5, 25% (w/v) PEG 3350