7T5W

Structure of E. coli CapH C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00004
Spacegroup name	P 1 21 1
Unit cell lengths	44.023, 39.855, 46.256
Unit cell angles	90.00, 98.73, 90.00

Refinement procedure

Resolution	45.720 - 1.750
R-factor	0.2112
Rwork	0.210
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7t5v
RMSD bond length	0.010
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.780
High resolution limit [Å]	1.750	1.750
Rmeas	0.070	0.784
Rpim	0.039	0.450
Number of reflections	15936	768
<I/σ(I)>	26.2	1.1
Completeness [%]	99.5
Redundancy	3.2
CC(1/2)	1.000	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Sodium Citrate pH 3.0, 1.6 M LiSO4