7T5M
Structure of HLA-A*02:01-FLPTPEELGLLGPPRPQVLA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 70.178, 99.903, 135.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.800 - 1.670 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gso |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rpim | 0.041 | 0.351 |
| Number of reflections | 111273 | 5154 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.998 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 0.12M Monosaccharides (D-Glucose; D-Mannose; D-Galactose; L-Fucose; D-Xylose; N-Acetyl-D-Glucosamine), 0.1M of Na-HEPES and MOPS buffer at pH 7.5, 20% W/V Glycerol and 10% W/V PEG 4000 |
