7T5G

Structure of rabies virus phosphoprotein C-terminal domain, S210E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	44.704, 69.089, 75.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.490 - 1.700
R-factor	0.2065
Rwork	0.204
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyi
RMSD bond length	0.002
RMSD bond angle	0.441
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.490	1.868
High resolution limit [Å]	1.696	1.696
Rmerge	0.111	1.680
Rmeas	0.116	1.757
Rpim	0.032	0.505
Number of reflections	14479	724
<I/σ(I)>	13.086	1.862
Completeness [%]	54.2	10.94
Redundancy	12.91	11.35
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.75	281	1.9 M ammonium sulfate, 0.2 M potassium sodium tartrate and 0.1 M sodium citrate