7T5C
X-ray structure of Neurospora crassa Polysaccharide Monooxygenase 9D (NcLPMO9D) at low pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-04 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.668, 42.210, 69.715 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 12.610 - 1.500 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tkh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.610 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.142 | 0.278 |
| Rmeas | 0.149 | 0.313 |
| Number of reflections | 62507 | 6159 |
| <I/σ(I)> | 62.56 | 7.14 |
| Completeness [%] | 99.8 | 99.97 |
| Redundancy | 8.2 | 4.5 |
| CC(1/2) | 0.992 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.4 | 295 | PEG 3350, HEPES |
