7T50
Crystal structure of the molybdate-binding periplasmic protein ModA from the bacteria Pseudomonsa aeruginosa in chromate-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.627, 41.223, 111.366 |
| Unit cell angles | 90.00, 92.76, 90.00 |
Refinement procedure
| Resolution | 41.480 - 1.900 |
| R-factor | 0.2369 |
| Rwork | 0.235 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.570 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.103 | 0.751 |
| Rmeas | 0.122 | 0.888 |
| Rpim | 0.065 | 0.469 |
| Number of reflections | 1972 | |
| <I/σ(I)> | 8.6 | 1.7 |
| Completeness [%] | 99.1 | 94.7 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.996 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Tris-HCl pH 8.5, 2.1 M ammonium sulfate, 20% v/v glycerol |
