7T3X
Structure of unphosphorylated Pediculus humanus (Ph) PINK1 D334A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 65 |
| Unit cell lengths | 116.118, 116.118, 70.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.830 - 3.530 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eqi |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.502 |
| Data reduction software | XDS (Nov 1, 2016) |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.830 | 3.870 |
| High resolution limit [Å] | 3.530 | 3.530 |
| Rmerge | 0.410 | 0.953 |
| Rpim | 0.188 | |
| Number of reflections | 6769 | 1609 |
| <I/σ(I)> | 4.2 | 2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281 | 20% polyethylene glycol 3350, 0.2 M triammonium citrate, 0.1 M ammonium sulphate, 0.01 M magnesium chloride |
