7T3S

Crystal Structure of Mouse Cadherin-23 EC8-9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	41.827, 56.194, 93.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.213 - 2.966
Rwork	0.214
R-free	0.29920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tfl 5tfk
RMSD bond length	0.011
RMSD bond angle	1.442
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.213	3.020
High resolution limit [Å]	2.966	2.970
Number of reflections	4947	224
<I/σ(I)>	7.75	1.77
Completeness [%]	98.9	95.3
Redundancy	8.8	4.5
CC(1/2)	0.966	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M NaCl 20% PEG 3350