7T2A

Crystal structure of HLA-DP4 in complex with Ply

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	P 41 21 2
Unit cell lengths	101.669, 101.669, 215.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.400 - 3.040
R-factor	0.2194
Rwork	0.217
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p5k
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.400	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	22483	2197
<I/σ(I)>	8	1.5
Completeness [%]	99.8
Redundancy	2	2
CC(1/2)	0.996	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Sodium chloride, PEG 3350