7T27

Structure of phage FBB1 anti-CBASS nuclease Acb1-3'3'-cGAMP complex in post reaction state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-09-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	43.845, 43.845, 154.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.170 - 1.200
R-factor	0.1768
Rwork	0.176
R-free	0.19720
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.138
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.850	1.220
High resolution limit [Å]	1.200	1.200
Rpim	0.013	0.438
Number of reflections	47842	2307
<I/σ(I)>	23.2	1.4
Completeness [%]	99.1	94.9
Redundancy	16.9
CC(1/2)	1.000	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2 M ammonium sulfate, 0.1 M sodium citrate pH 4.6