7T1Y

Structure of the Fbw7-Skp1-MycCdegron complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	232.538, 232.538, 107.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.980 - 2.550
R-factor	0.2535
Rwork	0.253
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ovp
RMSD bond length	0.010
RMSD bond angle	1.396
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Number of reflections	48054	2297
<I/σ(I)>	19.11
Completeness [%]	99.7
Redundancy	9.5	6.2
CC(1/2)	0.995	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298.15	0.1 M Bicine pH6.5, 1.6 M Li2SO4