7T1S

Crystal structure of a bacterial sterol transporter

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-09-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97896, 0.91837, 0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	55.083, 57.257, 85.825
Unit cell angles	90.00, 97.26, 90.00

Refinement procedure

Resolution	28.630 - 1.910
R-factor	0.174
Rwork	0.172
R-free	0.20700
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.614
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.630	1.978
High resolution limit [Å]	1.910	1.910
Number of reflections	80757	4084
<I/σ(I)>	11.09
Completeness [%]	99.9
Redundancy	4.9
CC(1/2)	0.994	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3500, ammonium citrate dibasic