7T1M

Crystal structure of a bacterial sterol transporter

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97896, 0.91837, 0.97930
Spacegroup name	P 21 21 21
Unit cell lengths	39.882, 40.355, 142.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.820 - 1.600
R-factor	0.1998
Rwork	0.198
R-free	0.21970
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.820	1.657
High resolution limit [Å]	1.600	1.600
Number of reflections	31071	3061
<I/σ(I)>	30.96
Completeness [%]	97.8
Redundancy	19.5
CC(1/2)	1.000	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG3500; Bis-tris