7T1J

Crystal structure of RUBISCO from Rhodospirillaceae bacterium BRH_c57

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X17B1
Synchrotron site	NSLS
Beamline	X17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.907936
Spacegroup name	P 1 21 1
Unit cell lengths	74.807, 104.978, 369.611
Unit cell angles	90.00, 93.04, 90.00

Refinement procedure

Resolution	29.520 - 1.960
R-factor	0.1959
Rwork	0.196
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9rub
RMSD bond length	0.005
RMSD bond angle	0.620
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	phenix.refine (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.520	2.030
High resolution limit [Å]	1.960	1.960
Number of reflections	399609	35839
<I/σ(I)>	4.81
Completeness [%]	98.2
Redundancy	1.9
CC(1/2)	0.982	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Magnesium Formate pH 5.9, 20 % PEG 3,350