7T1C

Crystal structure of RUBISCO from Sulfurivirga caldicuralii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
Unit cell lengths	133.056, 133.056, 112.456
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.550 - 1.730
R-factor	0.1667
Rwork	0.166
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ius
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.550	1.790
High resolution limit [Å]	1.730	1.730
Number of reflections	61467	6044
<I/σ(I)>	15.9
Completeness [%]	100.0
Redundancy	19.4
CC(1/2)	0.999	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05 M Citric acid, 0.05 M Bis-TRIS propane pH 5.0, 16 % PEG 3,350