7T1A

Rev1 Ternary Complex with dATP and Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-15
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	62.136, 73.336, 115.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.650 - 1.810
R-factor	0.1951
Rwork	0.193
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6x6z
RMSD bond length	0.013
RMSD bond angle	1.278
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	55331	2406
<I/σ(I)>	12.53
Completeness [%]	99.3
Redundancy	3.5
CC(1/2)	0.997	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15-23% PEG3350, 200 mM ammonium nitrate