7T15
Hexameric SIVcpz CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91731 |
| Spacegroup name | P 63 |
| Unit cell lengths | 90.669, 90.669, 116.785 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.250 - 2.050 |
| R-factor | 0.2032 |
| Rwork | 0.202 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h47 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.491 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.520 | 78.520 | 2.110 |
| High resolution limit [Å] | 2.050 | 8.940 | 2.050 |
| Rmerge | 0.079 | 0.034 | 0.963 |
| Rmeas | 0.084 | 0.038 | 1.018 |
| Rpim | 0.027 | 0.014 | 0.330 |
| Total number of observations | 321339 | 4024 | 25139 |
| Number of reflections | 34170 | 439 | 2658 |
| <I/σ(I)> | 12.8 | 37 | 2.3 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 9.4 | 9.2 | 9.5 |
| CC(1/2) | 0.987 | 0.973 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 290 | Crystallant: 4.5% PEG 550MME, 0.15M KSCN, 0.1M Tris (pH 9.0), 4% 2,5-hexanediol. Crystals grew in 200 nL protein (12 mg/ml) + 200 nL crystallant. Cryoprotected in 20% (v/v) MPD |
