7T14
Hexameric SIVmac CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 6 |
| Unit cell lengths | 153.102, 153.102, 62.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.290 - 2.250 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h47 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.906 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.8) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.290 | 29.290 | 2.320 |
| High resolution limit [Å] | 2.250 | 8.990 | 2.250 |
| Rmerge | 0.161 | 0.033 | 0.724 |
| Rmeas | 0.180 | 0.037 | 0.805 |
| Rpim | 0.078 | 0.016 | 0.348 |
| Total number of observations | 206402 | 3436 | 18302 |
| Number of reflections | 39855 | 654 | 3516 |
| <I/σ(I)> | 9.7 | 34.8 | 2.6 |
| Completeness [%] | 99.4 | 97.3 | 95.6 |
| Redundancy | 5.2 | 5.3 | 5.2 |
| CC(1/2) | 0.994 | 0.999 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | Crystallant: 10% (w/v) PEG 6000, 5% (w/v) MPD, 100 mM HEPES (pH 7.5). Crystals grew in 200 nL protein (12 mg/ml) + 200 nL crystallant. Cryoprotected in 20% (v/v) MPD |
