7T12
Hexameric HIV-1 (O-group) CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.200, 151.730, 120.420 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 75.860 - 3.000 |
| R-factor | 0.2166 |
| Rwork | 0.214 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h47 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.860 | 75.860 | 3.080 |
| High resolution limit [Å] | 3.000 | 13.080 | 3.000 |
| Rmerge | 0.170 | 0.114 | 1.208 |
| Rmeas | 0.193 | 0.129 | 1.369 |
| Rpim | 0.088 | 0.056 | 0.626 |
| Total number of observations | 295052 | 3832 | 21300 |
| Number of reflections | 59020 | 752 | 4580 |
| <I/σ(I)> | 5.9 | 9.2 | 1.8 |
| Completeness [%] | 97.7 | 98 | 97.5 |
| Redundancy | 5 | 5.1 | 4.7 |
| CC(1/2) | 0.908 | 0.975 | 0.282 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | Crystallant: 10% (w/v) PEG 6000, 100 mM HEPES (pH 7.0), 100 mM Glycine. Crystals grew in 2 ul protein (40 mg/ml) + 2 ul crystallant. Cryoprotected in 40% (w/v) glucose |
