7SZX

Structure of the N-terminal nuclease and origin binding domain of Human Parvovirus B19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 32 2 1
Unit cell lengths	106.710, 106.710, 59.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.080 - 3.500
R-factor	0.2658
Rwork	0.262
R-free	0.29910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6usm
RMSD bond length	0.004
RMSD bond angle	0.867
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.080	3.625
High resolution limit [Å]	3.500	3.500
Rmerge	0.076	0.423
Rmeas	0.107	0.598
Rpim	0.076	0.423
Number of reflections	5099	488
<I/σ(I)>	5.37	1.67
Completeness [%]	98.7	97.41
Redundancy	2	2
CC(1/2)	0.993	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	2.5 M sodium chloride 0.1 M Tris-HCl pH 7.0 200 mM MgCl2