7SZO

Structure of a bacterial fimbrial tip containing FocH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-24
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.98
Spacegroup name	H 3 2
Unit cell lengths	214.777, 214.777, 532.051
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	176.780 - 2.800
R-factor	0.2464
Rwork	0.245
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb 3jwn
RMSD bond length	0.006
RMSD bond angle	0.910
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.5.0047)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	176.780	176.780	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.139	0.058	0.769
Number of reflections	109868	11415	9804
<I/σ(I)>	8.5
Completeness [%]	94.4	94.9	85
Redundancy	4.2	4.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M POTASSIUM CHLORIDE, 0.1 M REMARK 280 SODIUM CITRATE, PH 4.1