7SZL

Crystal structure of the Toll/interleukin-1 receptor domain of human IL-1R10 (IL-1RAPL2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 43 21 2
Unit cell lengths	50.655, 50.655, 181.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.880 - 2.300
R-factor	0.2396
Rwork	0.238
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3g
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.880	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	11338	1066
<I/σ(I)>	25.5
Completeness [%]	99.6	100
Redundancy	24.8	27
CC(1/2)	1.000	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2.4 M sodium malonate, pH 5.0