7SZ8

Crystal structure of human CELSR1 EC4-7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-08
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 1
Unit cell lengths	42.384, 76.943, 92.742
Unit cell angles	89.83, 87.78, 76.23

Refinement procedure

Resolution	46.378 - 2.337
Rwork	0.213
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t9t 5szn 5v5x 6c13 6e6b 6vg4
RMSD bond length	0.010
RMSD bond angle	1.752
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.378	2.390
High resolution limit [Å]	2.337	2.337
Number of reflections	47823	1953
<I/σ(I)>	15.375	8.824
Completeness [%]	85.3	82.5
Redundancy	3.3	3.2
CC(1/2)	0.992	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M magnesium chloride, 0.1 M MES pH 7.3, 30% PEG400